Requirement for bivalent cations in the actions of insulin and sodium nitroprusside on metabolism in rat adipocytes.
نویسندگان
چکیده
The requirement for Ca2+ and Mg2+ in the actions of insulin and sodium nitroprusside on rat adipocyte metabolism was investigated: sodium nitroprusside, but not insulin, increased cGMP levels in cells incubated in the absence of Ca2+ and/or Mg2+; sodium nitroprusside and insulin are unable to increase the incorporation of [14C]glucose into triglycerides and [14C]leucine into proteins in the absence of Ca2+ and Mg2+; sodium nitroprusside and insulin showed antilipolytic actions in Ca2+- and Mg2+-free medium. We conclude that in the absence of Ca2+ and Mg2+, sodium nitroprusside and insulin have very similar regulatory properties on triglyceride, protein synthesis and adrenaline-stimulated lipolysis, but not on cGMP levels in rat adipocytes. This could provide evidence that omission of bivalent cations was inhibitory at more than one site, or that sodium nitroprusside mimics insulin's actions by another mechanism that does not involve cGMP.
منابع مشابه
Effect of different concentrations of Zinc and their interaction with Sodium nitroprusside (SNP) on physiological and biochemical parameters of Plantago major L. Sara Nasiri Savadkoohi1*, Sakineh Saeidi-sar2 Abbas Ali Dehpour3 and Hossein Abbaspour1
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ورودعنوان ژورنال:
- FEBS letters
دوره 200 1 شماره
صفحات -
تاریخ انتشار 1986